Cultivation of wild indigenous Agaricus bisporus and Agaricus subrufescens from Pakistan
نویسندگان
چکیده
منابع مشابه
Spawn Cryopreservation of Agaricus Bisporus and A. Subrufescens Strains
One of the main problems for conservation of mushroom germplasm is that the traditional method of subculturing facilitates aging and contamination of cultures. The conservation of strains at ultra-low temperature in liquid nitrogen (-196 °C) is a widely used method, however, this method become expensive in collections with large number of strains and requires highly specialized personnel. The a...
متن کاملThe Genetic Linkage Map of the Medicinal Mushroom Agaricus subrufescens Reveals Highly Conserved Macrosynteny with the Congeneric Species Agaricus bisporus
Comparative linkage mapping can rapidly facilitate the transfer of genetic information from model species to orphan species. This macrosynteny analysis approach has been extensively used in plant species, but few example are available in fungi, and even fewer in mushroom crop species. Among the latter, the Agaricus genus comprises the most cultivable or potentially cultivable species. Agaricus ...
متن کاملVirus-Enriched Fraction from Agaricus bisporus
Partially purified virus preparations from sporophores of Agaricus bisporus affected with LaFrance disease had up to a 15-fold-higher RNA-dependent RNA polymerase activity than did comparable preparations from healthy sporophores. Enzyme activity was dependent upon the presence of Mg2+ and the four nucleoside triphosphates and was insensitive to actinomycin D, a-amanitin, and rifampin. The 3H-l...
متن کاملAgrobacterium tumefaciens mediated transformation of Agaricus bisporus
Agrobacterium tumefaciens is known to transfer parts of its tumor-inducing plasmid to filamentous fungi including Agaricus bisporus. We have used this transformation system to transform germinating basidiospores of A. bisporus. Analysis of hygromycin B-resistant clones showed that the T-DNA integrated at random sites and in multiple copies into the host genome. Furthermore we have been able to ...
متن کاملCrosses among Homokaryons from Commercial and Wild-Collected Strains of the Mushroom Agaricus brunnescens (= A. bisporus).
A new technique for the production of hybrid strains of the cultivated mushroom Agaricus brunnescens is described. Homokaryons were recovered from regenerated protoplasts obtained from several heterokaryotic strains. A total of 16 novel hybrids were produced in 63 attempted crosses between paired homokaryons. Recovery of both homokaryons and hybrids was verified by analysis of restriction fragm...
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ژورنال
عنوان ژورنال: Current Research in Environmental & Applied Mycology
سال: 2020
ISSN: 2229-2225
DOI: 10.5943/cream/10/1/36